Insertion junction: CLIP2.034931_3

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.034931
Chromosome:chromosome 7
Location:4663403
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre07.g344800 TNP21,CSB33 (1 of 70) PF07282 - Putative transposase DNA-binding domain (OrfB_Zn_ribbon); {"Probable transposon-derived protein of Chlamydomonas-Specific family B intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCATGCAGACCTGCTACAACTGCGGGTGTCAGGAGATGGCCATCAAGCGG
Internal bar code:TAACTATTTGCGAATCTTATTT

Confirmation - LEAP-Seq

LEAP-Seq distance:3151
LEAP-Seq percent confirming:93.5484
LEAP-Seq n confirming:29
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:31

Suggested primers for confirmation by PCR

Suggested primer 1:CGTTATCAACCCAGCCACCT
Suggested primer 2:GCAGCTGGATGAGGAGTTGT