Insertion junction: CLIP2.034940_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.034940
Chromosome:chromosome 1
Location:5979917
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g042450 (1 of 14) IPR006626//IPR011050 - Parallel beta-helix repeat // Pectin lyase fold/virulence factor CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGTCGAGGCCGACTACGAAGGATACCAGGACGCGGCTGGAACCACCCTGT
Internal bar code:CCCTGAGAGGAAGGCCTCCGAT

Confirmation - LEAP-Seq

LEAP-Seq distance:4290
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:72
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:72

Suggested primers for confirmation by PCR

Suggested primer 1:CGCAAACGAAGACTGCTAGC
Suggested primer 2:TACTGAACCCTGCGTGGAAC