| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.034971 |
| Chromosome: | chromosome 1 |
| Location: | 6358173 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g045000 | PRL9 | Predicted extracellular protein; (1 of 2) IPR001283//IPR002413//IPR014044 - Cysteine-rich secretory protein, allergen V5/Tpx-1-related // Ves allergen // CAP domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCACGGCACCATGCTCTCCGCTGCGCCGCACACCCCACACGCGCACCTGT |
| Internal bar code: | CTGATAGCGGTAGTGTGCCATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1595 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 60 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGTTTCGTCTCAGGTGAGGC |
| Suggested primer 2: | GTGCTGCCTTTGTGTCACTG |