Insertion junction: CLIP2.034978_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.034978
Chromosome:chromosome 9
Location:4379204
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g400293 (1 of 10) IPR029068 - Glyoxalase/Bleomycin resistance protein/Dihydroxybiphenyl dioxygenase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGGGCTGCATTCACGCCTACCACGCACACGCCATGTCCCTTCCTGCACC
Internal bar code:CCGGGGCTTTGTACCTTGAATC

Confirmation - LEAP-Seq

LEAP-Seq distance:2567
LEAP-Seq percent confirming:98.2759
LEAP-Seq n confirming:57
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:58

Suggested primers for confirmation by PCR

Suggested primer 1:TCCTGCCATGAACTCCACAC
Suggested primer 2:GTAGGAAGCAGCAGGAAGGG