| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.034981 |
| Chromosome: | chromosome 3 |
| Location: | 2383730 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g158464 | (1 of 1) PTHR13561//PTHR13561:SF26 - DNA REPLICATION REGULATOR DPB11-RELATED // F5A8.9 PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCTCTTCACGCGGCGCCCCCGGCTCCGCCTCTGGCAGCGGCTGCTCCTG |
| Internal bar code: | GATCCAGTGAAAATTGTGGTAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2894 |
| LEAP-Seq percent confirming: | 91.0256 |
| LEAP-Seq n confirming: | 71 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 78 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCTGCCTGTGGTACAAGA |
| Suggested primer 2: | AACAAAGCCGAGCGACATTG |