| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.034984 |
| Chromosome: | chromosome 9 |
| Location: | 3312548 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g395288 | NHA1 | (1 of 1) PTHR10283:SF103 - GENOMIC DNA, CHROMOSOME 3, P1 CLONE: MLD14-RELATED; Sodium ion/proton transporter protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCTTCTGGTGCCAATGGCTGCGTTGCCTATCCCCCATCCCCTCCCACC |
| Internal bar code: | GGGATATCGTCCTATTGTCAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1002 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 32 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGGTAAGGGTGCGAGTGTG |
| Suggested primer 2: | GATGGGGTTGGGGGAAAGAG |