| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.034986 |
| Chromosome: | chromosome 6 |
| Location: | 3149385 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g275800 | HTB31,HTB4,HTB3 | (1 of 27) K11252 - histone H2B (H2B); Histone H2B | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGCCTTCTTGGAGGGCTCCTTCTTGGCCTTCTTGGCGGCCTTCTCGGC |
| Internal bar code: | TTATGCGAGTGGAATCCAAAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2276 |
| LEAP-Seq percent confirming: | 84.7826 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGCCCTTCTTCAGGTAGCG |
| Suggested primer 2: | GTACACAGTACCACCCTGGC |