Insertion junction: CLIP2.034987_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.034987
Chromosome:chromosome 16
Location:1353329
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g651850 (1 of 3) PTHR13367:SF8 - OTU DOMAIN-CONTAINING PROTEIN 7B CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CAAATGCCACCTCGAAGTTGAAGCTGCCAAAGGTGCGGACGTACAGCGGA
Internal bar code:GCACTCGCGTGGGCTTGTCCTT

Confirmation - LEAP-Seq

LEAP-Seq distance:3955
LEAP-Seq percent confirming:98.7179
LEAP-Seq n confirming:77
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:78

Suggested primers for confirmation by PCR

Suggested primer 1:GACCTTCCAGGACAACCCAG
Suggested primer 2:TCAACGACCAGTAGCTGCAG