| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.035032 |
| Chromosome: | chromosome 3 |
| Location: | 5257278 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g182200 | (1 of 1) IPR001054//IPR028082//IPR029787 - Adenylyl cyclase class-3/4/guanylyl cyclase // Periplasmic binding protein-like I // Nucleotide cyclase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTATCCCCGCTACACCCCTGCGTAGCCGCCACCGCACACCTTTTCGTGC |
| Internal bar code: | AGCATGGGATTGTATCGTCACC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2200 |
| LEAP-Seq percent confirming: | 95.4545 |
| LEAP-Seq n confirming: | 63 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTACGGCATAGAGGAAGGCC |
| Suggested primer 2: | TTCTCATGGTCTGCAACGCT |