Insertion junction: CLIP2.035041_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.035041
Chromosome:chromosome 16
Location:1389014
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g652100 MAP2 (1 of 1) PTHR10804//PTHR10804:SF9 - PROTEASE FAMILY M24 METHIONYL AMINOPEPTIDASE, AMINOPEPTIDASE P // METHIONINE AMINOPEPTIDASE 2; Methionine aminopeptidase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACGAGGTGGAGCTGGACGGGCGCACGCACCAGGTCAAGTGCATCCGCAA
Internal bar code:GAGTGTTGCTCTTGTGCGTGCT

Confirmation - LEAP-Seq

LEAP-Seq distance:110
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:TAGTCGCATTCTACGGACGC
Suggested primer 2:TCAACCCCAAATACGACCCG