| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.035048 |
| Chromosome: | chromosome 14 |
| Location: | 1261284 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g616151 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGAAGAGGGGGTTAGGAGGACTGGGGCATGGCCGCAGGGGGGGGGTAAG |
| Internal bar code: | CGTAGACTGTAAGTATTGGGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3427 |
| LEAP-Seq percent confirming: | 81.0811 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCGCTGTATTCTTTCTGC |
| Suggested primer 2: | TCCCCTCCCTCAGTCATAGC |