Insertion junction: CLIP2.035055_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.035055
Chromosome:chromosome 3
Location:4965998
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g179300 SRH6 (1 of 1) K11367 - chromodomain-helicase-DNA-binding protein 1 [EC:3.6.4.12] (CHD1); SNF2-related DNA/RNA helicase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACAAACACACTCGCGCACACACACTTTGCGCAATGTTTTCCTTGTGCTC
Internal bar code:ACATGAGTCAGTGGTGTGCCGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1998
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:TATTGTCGGTGGTGGTCAGC
Suggested primer 2:ACCGTCTCCAGGGAGTACTC