| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.035071 |
| Chromosome: | chromosome 2 |
| Location: | 6489761 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388800 | GDH1 | Glutamate dehydrogenase; (1 of 2) 1.4.1.3 - Glutamate dehydrogenase (NAD(P)(+)) / Glutamic dehydrogenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTTGCGTGCGCCCATAAACGATTGTGCGTATGTGCACGGTACATATTTGG |
| Internal bar code: | TTCGCCGTAATAAGTGTAGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2400 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTCGAGTGGGTGCAGAAC |
| Suggested primer 2: | CAAGCACCTGGTCAACAAGC |