| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.035084 |
| Chromosome: | chromosome 7 |
| Location: | 3525414 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g336300 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAACCCGCCCCCATTCACGGTGGGCCAAATCGGCTGCCGGTCCGCAACA |
| Internal bar code: | TATTCAATCTCATTATATTGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1966 |
| LEAP-Seq percent confirming: | 73.6842 |
| LEAP-Seq n confirming: | 42 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 57 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGGGGTTTTCACGTGACG |
| Suggested primer 2: | GGGTGAAAAATGTGCGCTGT |