| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.035091 |
| Chromosome: | chromosome 13 |
| Location: | 1429041 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g572050 | SMG1 | (1 of 1) K08873 - PI-3-kinase-related kinase SMG-1 (SMG1); Nonsense-mediated decay (NMD) pathway protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCGCGGAGCTGCTGCGCCGCCGCGAGGCCTTGCTGGGCGGCGCGCTGG |
| Internal bar code: | GGGTAAGGAAGGAGATTATATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4892 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCTCCAATGCTCCCACTGAC |
| Suggested primer 2: | CTGGCATGTTTGGGTAGGGT |