| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.035113 |
| Chromosome: | chromosome 10 |
| Location: | 5104168 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g455190 | (1 of 1) PF04989 - Cephalosporin hydroxylase (CmcI) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GATTCAGCGGCGGCGGCGGCGGTGACAGGCGCGGTGGCAAGCCGCAGCCT |
| Internal bar code: | TTCGAAGGGATTCCATGCACTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 279 |
| LEAP-Seq percent confirming: | 68.1818 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGAGGGTTCCATGCATGA |
| Suggested primer 2: | TGTTTGCCAATGTGTGCTCG |