| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.035126 |
| Chromosome: | chromosome 6 |
| Location: | 8291269 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g307012 | (1 of 5) PF13391 - HNH endonuclease (HNH_2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTCATCGCCAGGCATACAGCTCCTGGCACGTACGCCAGGAGCTAGACCT |
| Internal bar code: | CTGGGTTCGCCTCCTAGGTTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2982 |
| LEAP-Seq percent confirming: | 65.2174 |
| LEAP-Seq n confirming: | 30 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAACTGGCGGTGAGTTGTTG |
| Suggested primer 2: | AGGCGGATTCTCCTACCCTT |