| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.035140 |
| Chromosome: | chromosome 3 |
| Location: | 4049417 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g171950 | PPC2,PEPC2 | (1 of 2) K01595 - phosphoenolpyruvate carboxylase (ppc); Phosphoenolpyruvate carboxylase 2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGGGCAGGCAGGAGGTGAAGCACAGCGCCAGTCTCAGGATTGGTTTCG |
| Internal bar code: | GTATATCCTATCTCTACCTTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 297 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGACCCAAACCAACCAACCA |
| Suggested primer 2: | CACGGGCTTTCGTTTCGTTT |