| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.035167 |
| Chromosome: | chromosome 7 |
| Location: | 6135570 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g355800 | (1 of 58) PTHR12393//PTHR12393:SF6 - SPHINGOMYELIN PHOSPHODIESTERASE RELATED // SPHINGOMYELIN PHOSPHODIESTERASE 2 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTTGATGACTTCCCACTATGTCAACACGCACCTGCTTTCACCAGTGTG |
| Internal bar code: | GTAAAGTCCGAAGTTATTCTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1288 |
| LEAP-Seq percent confirming: | 12.5 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCTACGCACATCCAAACGA |
| Suggested primer 2: | CCCCACTCGTACTGGAGGTA |