Insertion junction: CLIP2.035173_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.035173
Chromosome:chromosome 2
Location:922888
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g079100 XPA1 DNA repair protein; (1 of 1) K10847 - DNA-repair protein complementing XP-A cells (XPA) CDS
Cre02.g079150 (1 of 1) PF03031//PF13671 - NLI interacting factor-like phosphatase (NIF) // AAA domain (AAA_33) outside_mRNA

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCAGGCCTTCGGCGTGGTGATATGCTCCCAGTGCAAGCGCGATGAACCG
Internal bar code:TTTCTTATCACATAATCAAACG

Confirmation - LEAP-Seq

LEAP-Seq distance:242
LEAP-Seq percent confirming:88.8889
LEAP-Seq n confirming:8
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:ATCAACATGAGCGCCAAGGA
Suggested primer 2:GGTGCACAGCAGTTGAAAGG