| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.035200 |
| Chromosome: | chromosome 9 |
| Location: | 5022333 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g405750 | CAH8 | (1 of 2) PTHR11002//PTHR11002:SF13 - CARBONIC ANHYDRASE // BETA CARBONIC ANHYDRASE 1; Carbonic anhydrase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGAGTACCTGTGGATTGGCTGCAGCGACTCCCGCGTGCCGGTGGGTGCG |
| Internal bar code: | GATAAATGTTCTATCCCGTCGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1299 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCAAGTCAGCAAGTCCCGAC |
| Suggested primer 2: | GAACCTATTTCGACACGCGC |