| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.035203 |
| Chromosome: | chromosome 2 |
| Location: | 327410 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g075150 | RPB2 | (1 of 1) K03010 - DNA-directed RNA polymerase II subunit RPB2 (RPB2, POLR2B); DNA-directed RNA polymerase II, 135 kDa polypeptide | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGCCGGGTGGTGGAGCCAACACGTGGGCACAGGGAGGAGGGTGCGGTGC |
| Internal bar code: | CCCGGGATTAGGCCAACCTAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 591 |
| LEAP-Seq percent confirming: | 91.6667 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGGGTAGGTGGGGTGATTG |
| Suggested primer 2: | GGAGGAGAAGACGGAGACCT |