Insertion junction: CLIP2.035220_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.035220
Chromosome:chromosome 11
Location:1331848
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre11.g467706 (1 of 1) PTHR10625//PTHR10625:SF117 - HISTONE DEACETYLASE // SUBFAMILY NOT NAMED intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCACCCTGCCACCCACCCACCCTGTCACCCCAGAACATCCTGCTCCAGC
Internal bar code:TTCGGCAGCGTGTGAAAATATA

Confirmation - LEAP-Seq

LEAP-Seq distance:596
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:ACACACACACACACGACCTT
Suggested primer 2:TCTCTGACCTCCGTTTGTGC