| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.035221 |
| Chromosome: | chromosome 14 |
| Location: | 1377499 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g616850 | (1 of 7) PF00582 - Universal stress protein family (Usp) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGCCGCTGCGCCGGCTTGGCGGCGGCGGGTGTGACGCACTACAGCACCGA |
| Internal bar code: | GACTGCGAGGTGTCGGCCCGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1380 |
| LEAP-Seq percent confirming: | 54.2857 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 16 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGGCCACCACCAGAAGTTT |
| Suggested primer 2: | GTTCCGGTTTGGTTTCAGGC |