Insertion junction: CLIP2.035231_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.035231
Chromosome:chromosome 12
Location:4591352
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g521750 NUP133 Nucleoporin 133; (1 of 2) PF08801 - Nup133 N terminal like (Nucleoporin_N) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCCCGCACCCCCTCCCCCCAACTGTCAACGTGCCACCCCCCCGGGCCAG
Internal bar code:GATGATCACGGAATTTTCATTA

Confirmation - LEAP-Seq

LEAP-Seq distance:295
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:GTGTGGTGCGTCACTGTTTC
Suggested primer 2:GTCGTGTGTGTGTGTGTGTG