| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.035243 |
| Chromosome: | chromosome 12 |
| Location: | 5631240 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g531450 | HPAT3 | Hydroxyproline O-arabinosyl transferase 3 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TAGGTGTGAGCGCCCGCAGCAACGCACGACCTACGTCAGCCATCCCTTGC |
| Internal bar code: | GACTAACTTATATTGTCTCCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 420 |
| LEAP-Seq percent confirming: | 4.34783 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 22 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATTATCTGTGCGGCAAGGC |
| Suggested primer 2: | GAAATCGCACCACTCATCGC |