| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.035249 |
| Chromosome: | chromosome 6 |
| Location: | 1810180 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g263450 | EEF1A3 | (1 of 3) K03231 - elongation factor 1-alpha (EEF1A); Putative eukaryotic translation initiation factor 1 alpha | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGGGGCTGCCAAAGAAGGCTACGACAGTCCGTTTAGACTTGTCGGCACCT |
| Internal bar code: | GAATGTTACAGGTCTGTGGTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2175 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGTAGACGACCTCAGCCATC |
| Suggested primer 2: | TAACTTCACCACCGCCATCC |