| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.035260 |
| Chromosome: | chromosome 13 |
| Location: | 864353 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g567400 | PDE16 | (1 of 20) 3.1.4.17 - 3',5'-cyclic-nucleotide phosphodiesterase / Cyclic AMP phosphodiesterase; 3'%252C5'-cyclic-nucleotide phosphodiesterase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCACACGGGGCAGCAAGGTGGATGCAGGCGGGGAGCGATGCGGGCGCTTT |
| Internal bar code: | TCACGTTAGAGTTAAATTTTGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3385 |
| LEAP-Seq percent confirming: | 98.3871 |
| LEAP-Seq n confirming: | 61 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCCAATCAAACTTGCTCGC |
| Suggested primer 2: | GGTCACAAAGCTTTTCGCGT |