Insertion junction: CLIP2.035261_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.035261
Chromosome:chromosome 1
Location:5218373
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g036451 SMM2 S-adenosyl-L-methionine-dependent methyltransferase; (1 of 228) IPR029063 - S-adenosyl-L-methionine-dependent methyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACACCCGCTCCCGCCCTCGCAGCCACATCCGCTACCACGAGGACGGCT
Internal bar code:GATGGCGCTGCGGTGGGGTTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:3105
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:33
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:GGTGTCCAGTCCGTCCATTT
Suggested primer 2:AGGTGAGGTCCATTGCCATG