Insertion junction: CLIP2.035261_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.035261
Chromosome:chromosome 1
Location:5218378
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g036451 SMM2 S-adenosyl-L-methionine-dependent methyltransferase; (1 of 228) IPR029063 - S-adenosyl-L-methionine-dependent methyltransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTCAGGGGTGGACGCAGAAGTTGAAGCGGCAGCGGCTGGAGAAGCGGGG
Internal bar code:ATCTGTCCTATAGTTCTTCTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:2760
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:38
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:AGGTGAGGTCCATTGCCATG
Suggested primer 2:GGTGTCCAGTCCGTCCATTT