Insertion junction: CLIP2.035266_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.035266
Chromosome:chromosome 9
Location:6025503
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g412550 (1 of 3) IPR000104//IPR005069 - Antifreeze protein, type I // Nucleotide-diphospho-sugar transferase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGAAAAGCACATCACTGCGGCAGGAGGCAAACGAAGAAGGGGTCAGCCA
Internal bar code:TTCGGGAGTCTACCCCTTAGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:778
LEAP-Seq percent confirming:66.6667
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GTATCGCCTGCCAATCCGTA
Suggested primer 2:CAAGCAACCTGGCAGAAACC