Insertion junction: CLIP2.035270_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.035270
Chromosome:chromosome 8
Location:3826980
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g381483 SDR15 (1 of 239) IPR016024 - Armadillo-type fold; Short-chain dehydrogenase/reductase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTGTGTCGTGTCAGGGGCGCCCTGCCGCGTTCAGCATAGTCATACACACA
Internal bar code:TTTAACCCGTGCGAAATTTACC

Confirmation - LEAP-Seq

LEAP-Seq distance:623
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:16
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCCGTTTAGTCCCTGCTC
Suggested primer 2:AGTCCATTGTGCTCGAACGT