| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.035274 |
| Chromosome: | chromosome 7 |
| Location: | 5219302 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g349100 | HEL39 | ATP-dependent RNA helicase; (1 of 1) K12813 - pre-mRNA-splicing factor ATP-dependent RNA helicase DHX16 (DHX16) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGACGGCTGGGCGGAGAGCAACTTCAGCACGCAGTGGTGCTTCGAGAAC |
| Internal bar code: | GGTGTGGATCATTCTAACCATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 550 |
| LEAP-Seq percent confirming: | 50.0 |
| LEAP-Seq n confirming: | 1 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGTTCTTGATGGTGCGGTA |
| Suggested primer 2: | GGATGGTTGGGTTCTCGTGT |