Insertion junction: CLIP2.035284_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.035284
Chromosome:chromosome 1
Location:4876818
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g033950 EXN2 3'-5' exonuclease with GRF zinc finger domain; (1 of 1) K18417 - ERI1 exoribonuclease 2 [EC:3.1.-.-] (ERI2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACTCCCAAGCAGGCAGCCACCCACCCAGTGACTCGACGCATGCCCGCAG
Internal bar code:CTGTTTATTAAATATTTTGGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:544
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GAAAGGACACAACACACGCC
Suggested primer 2:CTCCTGCTCCTCTCGTTGTC