Insertion junction: CLIP2.035285_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.035285
Chromosome:chromosome 6
Location:3034934
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g274600 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAACACACTACGGCACCCACCACTACCACGCAAACAAACGCACACGCGCG
Internal bar code:GAAGTCGTGAGTTGGCGGCGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:789
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:30
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:TCGGACATGTTCGACAAGGG
Suggested primer 2:GTGTGAAGGCGTGTGTGAAC