| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.035288 |
| Chromosome: | chromosome 5 |
| Location: | 2831926 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241250 | (1 of 1) IPR000104//IPR000253//IPR001357 - Antifreeze protein, type I // Forkhead-associated (FHA) domain // BRCT domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTGTCGGTTGGCAGCCTGCGGTCTTTGTGGCAGCAGCGGCAGGAGGAG |
| Internal bar code: | GCTTATTGTGTTAGCCTCTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2046 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 2 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CATCTCCCTCCACGTCATCG |
| Suggested primer 2: | TGGGAAGAAGAGCTGGAGGA |