Insertion junction: CLIP2.035291_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.035291
Chromosome:chromosome 16
Location:5360778
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g683450 AGP2 (1 of 4) 2.7.7.27 - Glucose-1-phosphate adenylyltransferase / ADP-glucose synthase; ADP-glucose pyrophosphorylase large subunit intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCGAATGCAAACGCAGCTGGACGCCCAGAACCGCATCAAGACATTTTCT
Internal bar code:CCAAGGCCAAATAGATTATTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:2275
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:25

Suggested primers for confirmation by PCR

Suggested primer 1:GCTGAGATACTGGTCGACGG
Suggested primer 2:TCCCTTTGCATCCCATTCCC