Insertion junction: CLIP2.035301_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.035301
Chromosome:chromosome 17
Location:1675357
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre17.g708000 FAP260 Membrane-associated Flagellar Associated Protein 260; (1 of 11) PF13426 - PAS domain (PAS_9) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGACCTCGATCAGGAAATTGGAGTAGGTGATGGTAAGGAACACGTCGTC
Internal bar code:GTAGATACAAATTCGGGCTTCA

Confirmation - LEAP-Seq

LEAP-Seq distance:2332
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:54
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:54

Suggested primers for confirmation by PCR

Suggested primer 1:CGCTGGCGAGTTAAGAATGC
Suggested primer 2:ATGTTCTTGCCCTCCAGCTC