| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.035308 |
| Chromosome: | plastome |
| Location: | 204718 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| CreCp.g802336 | orf854 | Wendy transposon | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAAGCCAGTGCCACGGCTTATTTAAATCAGGTGGAAGCTTCCGGGTTTTT |
| Internal bar code: | GTGGGCCCGCGTTGGGGTTCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1595 |
| LEAP-Seq percent confirming: | 96.0 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 50 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTTTTCCATCCGCTCCGACA |
| Suggested primer 2: | GAAGGGGAAGGAGGAAGCAG |