| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.035314 |
| Chromosome: | chromosome 16 |
| Location: | 1178435 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g650550 | FAP103 | Flagellar Associated Protein 103; (1 of 4) K00940 - nucleoside-diphosphate kinase (ndk, NME) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTAGGTCCAGACGAGCGCCAAGTCGGTCATGCAGAGTCAGGGGTGGGAGC |
| Internal bar code: | CTGCTTATTGGGGATTGTTGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2793 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCATCAGCTTCAGGCCCT |
| Suggested primer 2: | CCCCTCACCCATTAACACCC |