Insertion junction: CLIP2.035328_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.035328
Chromosome:chromosome 8
Location:3181489
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g376650 CSR5 Carbohydrate sulfotransferase-related 5; (1 of 6) 2.8.2.8 - [Heparan sulfate]-glucosamine N-sulfotransferase / PAPS:N-desulfoheparin sulfotransferase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACGGTAGGTGCCCAAGCGCCCAGCCAGCCAACCAGGCAGGCGGCAATCTA
Internal bar code:GGTCTCGAGACTCTGCTGGACA

Confirmation - LEAP-Seq

LEAP-Seq distance:2065
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:34
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:ACGGTACCTTCCATCGGAGA
Suggested primer 2:TCACACAGTCTGAACTCGCC