| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.035337 |
| Chromosome: | chromosome 14 |
| Location: | 989591 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g613850 | (1 of 1) K12585 - exosome complex exonuclease DIS3/RRP44 (DIS3, RRP44) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCAAAGATATCCAGGCCCAGGAGAGAGGGAGAGAAGGTGGAGGACTATG |
| Internal bar code: | TATTAGTTGGAAGTGGCTAGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1366 |
| LEAP-Seq percent confirming: | 95.4545 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACGCAAGACAAGGAGACA |
| Suggested primer 2: | TAAGGTGGGGGTATGCGGTA |