| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.035348 |
| Chromosome: | chromosome 12 |
| Location: | 2177138 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g509400 | RIR2L,RIR3,RIR2B | (1 of 2) K10808 - ribonucleoside-diphosphate reductase subunit M2 (RRM2); Ribonucleoside-diphosphate reductase R2-like subunit | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGGGGTAAGGGGAAGGTGAGATGAGAGGAAGGTGCCGTGGGTGACGGGG |
| Internal bar code: | CGGAGATATGTGCTGAGAAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4621 |
| LEAP-Seq percent confirming: | 92.7083 |
| LEAP-Seq n confirming: | 89 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 96 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGACGAGGACTTGCTACGAG |
| Suggested primer 2: | GAACACGTTGGAGGAGCTGA |