| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.035353 |
| Chromosome: | chromosome 2 |
| Location: | 4388720 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g105550 | (1 of 1) IPR000104//IPR003008 - Antifreeze protein, type I // Tubulin/FtsZ, GTPase domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAGGGGATCGAAAGCGAGTGCATGCACACGTTGGGTGCATTTACGTTA |
| Internal bar code: | AAGGATCTTATAATGATGCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3491 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 37 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTGCACGCTAACAAGCAAA |
| Suggested primer 2: | TACGCCACCTCAAGTGTCAC |