| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.035353 |
| Chromosome: | chromosome 2 |
| Location: | 4388721 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g105550 | (1 of 1) IPR000104//IPR003008 - Antifreeze protein, type I // Tubulin/FtsZ, GTPase domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGATCAAAAGGTGCTGTCGGGCGCGGACGTGGCGGCACATACGCTGGAGG |
| Internal bar code: | CGGAACAAAAGTGTTTTTCTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2744 |
| LEAP-Seq percent confirming: | 81.8182 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACGCCACCTCAAGTGTCAC |
| Suggested primer 2: | GGTGCACGCTAACAAGCAAA |