| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.035354 |
| Chromosome: | chromosome 12 |
| Location: | 3599049 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g512950 | CIT1 | (1 of 1) K07240 - chromate transporter (chrA); Chromate ion transporter | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACACACACACACACACACACACACACACACACACACACATGATGGCTGG |
| Internal bar code: | CAGAGCGTAAGGAGACGTTATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1252 |
| LEAP-Seq percent confirming: | 93.0233 |
| LEAP-Seq n confirming: | 40 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCTTCTTGACTGTGCCGAT |
| Suggested primer 2: | CCACACACACACACACACAC |