| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.035372 |
| Chromosome: | chromosome 14 |
| Location: | 3485749 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre14.g630600 | (1 of 12) IPR000104//IPR002893 - Antifreeze protein, type I // Zinc finger, MYND-type | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCGCCAGCCTGGGCCAGAGGCTGCTGCTGAAGGATTGTGATAAGTTGCT |
| Internal bar code: | AAACTTGAGCGATACACATTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2100 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 66 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTGTCACTTTCCCCGTTTGC |
| Suggested primer 2: | CTGCAACTTCCACATGGCAC |