| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.035378 |
| Chromosome: | chromosome 6 |
| Location: | 4944342 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g281200 | (1 of 1) K16743 - abnormal spindle-like microcephaly-associated protein (ASPM, ASP) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCCGCCAGGTAGCGGCTGCGCTGGCAGTGGCTGCGCCAGGCTGCCTGG |
| Internal bar code: | GGGTATCATCCAATGGACGGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2912 |
| LEAP-Seq percent confirming: | 98.0392 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 51 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCAAGCTGAGAAGCTGCGT |
| Suggested primer 2: | CATTAGCCAAACCGCAGCTC |