| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.035391 |
| Chromosome: | chromosome 6 |
| Location: | 5113960 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g282850 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCCGCCAGCAGCGTGGCTGCGCCTAGCAGCCTGACTGCGTCGCCGGCG |
| Internal bar code: | TTTCCAGTGAGGTTGTATCATA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3745 |
| LEAP-Seq percent confirming: | 67.6056 |
| LEAP-Seq n confirming: | 48 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 71 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATTTCGCCAACAACCAACGG |
| Suggested primer 2: | CAGCAGCAGCAAGACAAAGG |