Insertion junction: CLIP2.035429_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.035429
Chromosome:chromosome 6
Location:7261103
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g800731 (1 of 17) PF00415 - Regulator of chromosome condensation (RCC1) repeat (RCC1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCTCGCTGTCTAGCATCCAGCTCCGCGTCGTGTTACAGTGAATAGCCCCG
Internal bar code:TTATTACTAGGCTGGCTCGACG

Confirmation - LEAP-Seq

LEAP-Seq distance:1036
LEAP-Seq percent confirming:96.1538
LEAP-Seq n confirming:25
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:26

Suggested primers for confirmation by PCR

Suggested primer 1:GTCCACATTACGCAGTCCCA
Suggested primer 2:TGCTGCGTCTTCTGATGTGT